Production of Polyclonal Antibodies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein 1
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منابع مشابه
Production of Polyclonal Antibodies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein 1
The gene encoding the triple gene block protein 1 (TGBp1) of Potato mop-top virus (PMTV) was cloned into expression vector pQE32 tagging the protein with 6xHis on the N-terminus. When the gene was enshortened on its 3¢-end by two different restriction digestions, efficient and high yield bacterial expression was achieved in both cases, as shown by sodium dodecyl sulphate polyacrylamide gel elec...
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The cysteine-rich 8K protein of Potato mop-top virus (PMTV) suppresses host RNA silencing. In this study, evolutionary analysisof 8K sequences of PMTV isolates was studied on the basis of nucleotide and amino acid sequences. Twenty-one positively selected sites were identified in 8K codingregions. Recombination events were found in the 8K of PMTV isolates with a rate of 1.8. Totally 30 haplotyp...
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Functions of viral proteins can be regulated through phosphorylation by serine/threonine kinases in plants, but little is known about the involvement of tyrosine kinases in plant virus infection. In this study, TGBp3, one of the three movement proteins encoded by a triple gene block (TGB) of Potato mop-top virus (PMTV), was detected for the first time in PMTV-infected plants and found to be tyr...
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ژورنال
عنوان ژورنال: Journal of Phytopathology
سال: 2006
ISSN: 0931-1785,1439-0434
DOI: 10.1111/j.1439-0434.2006.01121.x